Journal: Journal of Nanobiotechnology

Article Title: Implantation of biomimetic polydopamine nanocomposite scaffold promotes optic nerve regeneration through modulating inhibitory microenvironment

doi: 10.1186/s12951-024-02962-y

Figure Lengend Snippet: Implantation of GA@PDA scaffold scavenged the accumulated ROS and down-regulated inflammation after ONC. (a) Fluorescence images of ROS in the optic nerve labeled with the fluorescence probe DCF (green) 3 weeks post scaffold implantation. DAPI (blue) was used to stain nuclei. (b-d) Immunofluorescence staining images and mean fluorescence intensity of IL-1β and TNF-α in the retina 3 weeks post scaffold implantation ( n = 5). (e-h) Western blot analysis of TNF-α, IL-1β and IL-6 in each group ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001, and ns, no significance

Article Snippet: The sections were washed three times in 10 mM PBS, blocked with PBS containing 0.3% Triton X-100 and 10% normal goat serum at RT for 1 h, and incubated overnight with the antibodies, including rabbit polyclonal anti-TNF-α antibody (1:500, Bioss, bs-10802R), mouse monoclonal anti-IL-1β antibody (1:500, Proteintech, 66737-1-Ig), rabbit polyclonal anti-GAP-43 antibody (1:200, Abcam, ab75810), mouse monoclonal anti-βIII Tubulin antibody (1:500, Promega, G7121), mouse monoclonal anti-CD206 antibody (1:500, abcam, ab64693), mouse monoclonal anti-CD86 antibody (1:500, abcam, ab220188), Rabbit monoclonal anti-iNOS antibody (1:500, abcam, ab178945) and Rabbit polyconal anti- Arg-1 antibody (1:500, proteintech, 16001-1-AP).

Techniques: Fluorescence, Labeling, Staining, Immunofluorescence, Western Blot